
Name:__________________________________ Grade & Sec.:_________ Score:________
Teacher:________________________________ Date:_________________ Rating:_______
Activity 1
Test for Carbohydrates and Lipids
Objective:
• To detect the presence of carbohydrates and lipids in food samples
using chemical tests.
Materials:
• Iodine solution or tincture of iodine
• Benedict’s solution
• food samples for testing carbohydrates ( cooked pasta, cracker,
• cooked rice, corn syrup, table sugar, pineapple)
• food samples for testing lipids (oil, peanut butter, egg, fried chicken,
• butter, milk, burger)
• 6 pcs.small test tubes or vials per group
• 6 pcs. test tube holders per group
• 2 pcs.droppers per group
• mortar & pestle per group
• 1 spot plate per group
Procedure:
A. Carbohydrates
Iodine Test for Starch
1. Place ½ teaspoon of each food sample on the well of a spot plate. Make sure
that the food samples are far from each other.
2. Add 3 drops of Lugol’s Iodine solution or tincture of iodine on each food
sample.
3. Note that Lugol’s iodine solution or tincture of iodine changes from yellow to
blue or black in the presence of starch.
4. Write your observation in Table A.
B. Benedict’s Test for Reducing Sugar
1. Place a pinch of the food samples to be tested into a test tube.
2. Add 1 full dropper of Benedict’s solution to each test tube.
3. Gently shake the test tube or vial.